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Nanolight品牌3011 Coelenterazine-SOL in vivo中英文說明書
3011 Coelenterazine-SOL in vivo說明
Native Coelenterazine formulated to be water-soluble and safe for in vivo imaging applications with Gaussia Luciferase.
Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water-soluble Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leADIng to high Gaussia luciferase signals.
| ?Content: | ?native Coelenterazine + non-toxic additives for water-solublity |
| Packaging size: | ?500 ug CTZ / vial in a freeze-dried form |
| ?Storage: | sealed vials can be stored at: -80°C for long-term storage -20°C for short-term storage Store dry and dark! |
| ?Application: | ?just add sterile water, vortex and use up to 500 ug CTZ in a single injection |
| Application example: |
A detailed describtion of this new water soluble Coelenterazine can be found in:
A Water-Soluble Coelenterazine for Sensitive In Vivo Imaging of Coelenterate Luciferases. Danielle Morse and Bakhos A Tannous |
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